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celltrace calcein red-orange dye  (Thermo Fisher)


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    Structured Review

    Thermo Fisher celltrace calcein red-orange dye
    Migration of activated PBT plated onto fresh tumor slices was analyzed in EGI-1 and MMTV-PyMT tumor model, whilst resident tumor-infiltrating T lymphocytes were analyzed in mPDAC and KPC tumor model. Illustrative images of T cell migration tracks in EGI-1, MMTV-PyMT, mPDAC, and KPC tumor models. Tumor stroma (fibronectin) in red, tumor cells (EpCAM in EGI-1, MMTV-PyMT, and KPC tumor models, CD44 in mPDAC tumor models), in blue and T cells (CD8 in mPDAC and KPC, <t>Calcein</t> in MMTV-PyMT, and EGI-1 tumor models) in green. Tracks are color-coded to illustrate track displacement. Scale bar = 100 µm. T cell migration speed, T cell displacement, and trajectory straightness in all tumor models. ***p - value>0.001, p-value>0.05, Student’s t-test. Results are shown as mean ± SD. Figure 5—source data 1. Source data file for .
    Celltrace Calcein Red Orange Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltrace+calcein+red-orange+dye/pmc08203293-369-18-22
    Average 90 stars, based on 1 article reviews
    celltrace calcein red-orange dye - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment"

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment

    Journal: eLife

    doi: 10.7554/eLife.58688

    Migration of activated PBT plated onto fresh tumor slices was analyzed in EGI-1 and MMTV-PyMT tumor model, whilst resident tumor-infiltrating T lymphocytes were analyzed in mPDAC and KPC tumor model. Illustrative images of T cell migration tracks in EGI-1, MMTV-PyMT, mPDAC, and KPC tumor models. Tumor stroma (fibronectin) in red, tumor cells (EpCAM in EGI-1, MMTV-PyMT, and KPC tumor models, CD44 in mPDAC tumor models), in blue and T cells (CD8 in mPDAC and KPC, Calcein in MMTV-PyMT, and EGI-1 tumor models) in green. Tracks are color-coded to illustrate track displacement. Scale bar = 100 µm. T cell migration speed, T cell displacement, and trajectory straightness in all tumor models. ***p - value>0.001, p-value>0.05, Student’s t-test. Results are shown as mean ± SD. Figure 5—source data 1. Source data file for .
    Figure Legend Snippet: Migration of activated PBT plated onto fresh tumor slices was analyzed in EGI-1 and MMTV-PyMT tumor model, whilst resident tumor-infiltrating T lymphocytes were analyzed in mPDAC and KPC tumor model. Illustrative images of T cell migration tracks in EGI-1, MMTV-PyMT, mPDAC, and KPC tumor models. Tumor stroma (fibronectin) in red, tumor cells (EpCAM in EGI-1, MMTV-PyMT, and KPC tumor models, CD44 in mPDAC tumor models), in blue and T cells (CD8 in mPDAC and KPC, Calcein in MMTV-PyMT, and EGI-1 tumor models) in green. Tracks are color-coded to illustrate track displacement. Scale bar = 100 µm. T cell migration speed, T cell displacement, and trajectory straightness in all tumor models. ***p - value>0.001, p-value>0.05, Student’s t-test. Results are shown as mean ± SD. Figure 5—source data 1. Source data file for .

    Techniques Used: Migration


    Figure Legend Snippet:

    Techniques Used: Derivative Assay, Recombinant, Cell Isolation, Activation Assay, Software

    Related Articles

    Migration:

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: To evaluate T cell migration in the EGI-1 model, activated human CD8+ T cells were first labeled with CellTrace Calcein red-orange dye (ThermoFischer).

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: To evaluate T cell migration in the EGI-1 model, activated human CD8+ T cells were first labeled with CellTrace Calcein red-orange dye (ThermoFischer).

    Labeling:

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: To evaluate T cell migration in the EGI-1 model, activated human CD8+ T cells were first labeled with CellTrace Calcein red-orange dye (ThermoFischer).

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: To evaluate T cell migration in the EGI-1 model, activated human CD8+ T cells were first labeled with CellTrace Calcein red-orange dye (ThermoFischer).

    Derivative Assay:

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: To evaluate T cell migration in the EGI-1 model, activated human CD8+ T cells were first labeled with CellTrace Calcein red-orange dye (ThermoFischer).

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: To evaluate T cell migration in the EGI-1 model, activated human CD8+ T cells were first labeled with CellTrace Calcein red-orange dye (ThermoFischer).

    Recombinant:

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: To evaluate T cell migration in the EGI-1 model, activated human CD8+ T cells were first labeled with CellTrace Calcein red-orange dye (ThermoFischer).

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: To evaluate T cell migration in the EGI-1 model, activated human CD8+ T cells were first labeled with CellTrace Calcein red-orange dye (ThermoFischer).

    Cell Isolation:

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: To evaluate T cell migration in the EGI-1 model, activated human CD8+ T cells were first labeled with CellTrace Calcein red-orange dye (ThermoFischer).

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: To evaluate T cell migration in the EGI-1 model, activated human CD8+ T cells were first labeled with CellTrace Calcein red-orange dye (ThermoFischer).

    Activation Assay:

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: To evaluate T cell migration in the EGI-1 model, activated human CD8+ T cells were first labeled with CellTrace Calcein red-orange dye (ThermoFischer).

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: To evaluate T cell migration in the EGI-1 model, activated human CD8+ T cells were first labeled with CellTrace Calcein red-orange dye (ThermoFischer).

    Software:

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: To evaluate T cell migration in the EGI-1 model, activated human CD8+ T cells were first labeled with CellTrace Calcein red-orange dye (ThermoFischer).

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: To evaluate T cell migration in the EGI-1 model, activated human CD8+ T cells were first labeled with CellTrace Calcein red-orange dye (ThermoFischer).



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    Thermo Fisher celltrace calcein red-orange dye
    Migration of activated PBT plated onto fresh tumor slices was analyzed in EGI-1 and MMTV-PyMT tumor model, whilst resident tumor-infiltrating T lymphocytes were analyzed in mPDAC and KPC tumor model. Illustrative images of T cell migration tracks in EGI-1, MMTV-PyMT, mPDAC, and KPC tumor models. Tumor stroma (fibronectin) in red, tumor cells (EpCAM in EGI-1, MMTV-PyMT, and KPC tumor models, CD44 in mPDAC tumor models), in blue and T cells (CD8 in mPDAC and KPC, <t>Calcein</t> in MMTV-PyMT, and EGI-1 tumor models) in green. Tracks are color-coded to illustrate track displacement. Scale bar = 100 µm. T cell migration speed, T cell displacement, and trajectory straightness in all tumor models. ***p - value>0.001, p-value>0.05, Student’s t-test. Results are shown as mean ± SD. Figure 5—source data 1. Source data file for .
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    Migration of activated PBT plated onto fresh tumor slices was analyzed in EGI-1 and MMTV-PyMT tumor model, whilst resident tumor-infiltrating T lymphocytes were analyzed in mPDAC and KPC tumor model. Illustrative images of T cell migration tracks in EGI-1, MMTV-PyMT, mPDAC, and KPC tumor models. Tumor stroma (fibronectin) in red, tumor cells (EpCAM in EGI-1, MMTV-PyMT, and KPC tumor models, CD44 in mPDAC tumor models), in blue and T cells (CD8 in mPDAC and KPC, <t>Calcein</t> in MMTV-PyMT, and EGI-1 tumor models) in green. Tracks are color-coded to illustrate track displacement. Scale bar = 100 µm. T cell migration speed, T cell displacement, and trajectory straightness in all tumor models. ***p - value>0.001, p-value>0.05, Student’s t-test. Results are shown as mean ± SD. Figure 5—source data 1. Source data file for .
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    Migration of activated PBT plated onto fresh tumor slices was analyzed in EGI-1 and MMTV-PyMT tumor model, whilst resident tumor-infiltrating T lymphocytes were analyzed in mPDAC and KPC tumor model. Illustrative images of T cell migration tracks in EGI-1, MMTV-PyMT, mPDAC, and KPC tumor models. Tumor stroma (fibronectin) in red, tumor cells (EpCAM in EGI-1, MMTV-PyMT, and KPC tumor models, CD44 in mPDAC tumor models), in blue and T cells (CD8 in mPDAC and KPC, <t>Calcein</t> in MMTV-PyMT, and EGI-1 tumor models) in green. Tracks are color-coded to illustrate track displacement. Scale bar = 100 µm. T cell migration speed, T cell displacement, and trajectory straightness in all tumor models. ***p - value>0.001, p-value>0.05, Student’s t-test. Results are shown as mean ± SD. Figure 5—source data 1. Source data file for .
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    Image Search Results


    Migration of activated PBT plated onto fresh tumor slices was analyzed in EGI-1 and MMTV-PyMT tumor model, whilst resident tumor-infiltrating T lymphocytes were analyzed in mPDAC and KPC tumor model. Illustrative images of T cell migration tracks in EGI-1, MMTV-PyMT, mPDAC, and KPC tumor models. Tumor stroma (fibronectin) in red, tumor cells (EpCAM in EGI-1, MMTV-PyMT, and KPC tumor models, CD44 in mPDAC tumor models), in blue and T cells (CD8 in mPDAC and KPC, Calcein in MMTV-PyMT, and EGI-1 tumor models) in green. Tracks are color-coded to illustrate track displacement. Scale bar = 100 µm. T cell migration speed, T cell displacement, and trajectory straightness in all tumor models. ***p - value>0.001, p-value>0.05, Student’s t-test. Results are shown as mean ± SD. Figure 5—source data 1. Source data file for .

    Journal: eLife

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment

    doi: 10.7554/eLife.58688

    Figure Lengend Snippet: Migration of activated PBT plated onto fresh tumor slices was analyzed in EGI-1 and MMTV-PyMT tumor model, whilst resident tumor-infiltrating T lymphocytes were analyzed in mPDAC and KPC tumor model. Illustrative images of T cell migration tracks in EGI-1, MMTV-PyMT, mPDAC, and KPC tumor models. Tumor stroma (fibronectin) in red, tumor cells (EpCAM in EGI-1, MMTV-PyMT, and KPC tumor models, CD44 in mPDAC tumor models), in blue and T cells (CD8 in mPDAC and KPC, Calcein in MMTV-PyMT, and EGI-1 tumor models) in green. Tracks are color-coded to illustrate track displacement. Scale bar = 100 µm. T cell migration speed, T cell displacement, and trajectory straightness in all tumor models. ***p - value>0.001, p-value>0.05, Student’s t-test. Results are shown as mean ± SD. Figure 5—source data 1. Source data file for .

    Article Snippet: To evaluate T cell migration in the EGI-1 model, activated human CD8+ T cells were first labeled with CellTrace Calcein red-orange dye (ThermoFischer).

    Techniques: Migration

    Journal: eLife

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment

    doi: 10.7554/eLife.58688

    Figure Lengend Snippet:

    Article Snippet: To evaluate T cell migration in the EGI-1 model, activated human CD8+ T cells were first labeled with CellTrace Calcein red-orange dye (ThermoFischer).

    Techniques: Derivative Assay, Recombinant, Cell Isolation, Activation Assay, Software